Somatic embryogenesis from Lycopersicon peruvianum leaf mesophyll protoplasts
Identifieur interne : 004783 ( Main/Exploration ); précédent : 004782; suivant : 004784Somatic embryogenesis from Lycopersicon peruvianum leaf mesophyll protoplasts
Auteurs : J. Zapata [États-Unis, Philippines] ; C. Sink [États-Unis]Source :
- Theoretical and Applied Genetics [ 0040-5752 ] ; 1981-09-01.
Abstract
Summary: One to five percent of Lycopersicon peruvianum (L.) Mill. leaf mesophyll protoplasts undergo cell division and concomitant organization to form embryogenic-like structures when cultured in Murashige and Skoog medium (1962) containing 3% sucrose, 9% mannitol, 1.0 mg/l kinetin (K) and 1.0 mg/l naphthalene acetic acid (NAA) at pH 5.6–5.8 (medium A). These embryogenic structures, after passing through developmental stages similar to those observed in zygotic embryogeny, are capable of forming shoots on hormone-free medium A. In medium B, wherein 0.5 mg/l of 2,4-dichlorophenoxyacetic (2,4-D) replaced the hormones (K and NAA), embryogenic structures did not develop. However, callus originating in medium B retained morphogenetic capacity as was evidenced by subsequent shoot regeneration when they were transferred to medium A with K and NAA replaced by 1.0 mg/l zeatin (Z). The potential value of incorporating this regeneration trait into Lycopersicon species and cultivated lines for use in tissue culture programs is discussed.
Url:
DOI: 10.1007/BF00264977
Affiliations:
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Zapata</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Horticulture, Michigan State University, East Lansing, Michigan</wicri:regionArea><placeName><region type="state">Michigan</region><settlement type="city">East Lansing</settlement></placeName><orgName type="university">Université d'État du Michigan</orgName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Philippines</country><wicri:regionArea>International Rice Research Institute, Los Banos, Laguna</wicri:regionArea><wicri:noRegion>Laguna</wicri:noRegion></affiliation></author><author><name sortKey="Sink, C" sort="Sink, C" uniqKey="Sink C" first="C." last="Sink">C. Sink</name><affiliation wicri:level="4"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Horticulture, Michigan State University, East Lansing, Michigan</wicri:regionArea><placeName><region type="state">Michigan</region><settlement type="city">East Lansing</settlement></placeName><orgName type="university">Université d'État du Michigan</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j">Theoretical and Applied Genetics</title><title level="j" type="sub">International Journal of Plant Breeding Research</title><title level="j" type="abbrev">Theoret. Appl. Genetics</title><idno type="ISSN">0040-5752</idno><idno type="eISSN">1432-2242</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1981-09-01">1981-09-01</date><biblScope unit="volume">59</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="265">265</biblScope><biblScope unit="page" to="268">268</biblScope></imprint><idno type="ISSN">0040-5752</idno></series><idno type="istex">5741DE330F1C71BB6598A62C48979AD7601BD4BD</idno><idno type="DOI">10.1007/BF00264977</idno><idno type="ArticleID">BF00264977</idno><idno type="ArticleID">Art1</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0040-5752</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: One to five percent of Lycopersicon peruvianum (L.) Mill. leaf mesophyll protoplasts undergo cell division and concomitant organization to form embryogenic-like structures when cultured in Murashige and Skoog medium (1962) containing 3% sucrose, 9% mannitol, 1.0 mg/l kinetin (K) and 1.0 mg/l naphthalene acetic acid (NAA) at pH 5.6–5.8 (medium A). These embryogenic structures, after passing through developmental stages similar to those observed in zygotic embryogeny, are capable of forming shoots on hormone-free medium A. In medium B, wherein 0.5 mg/l of 2,4-dichlorophenoxyacetic (2,4-D) replaced the hormones (K and NAA), embryogenic structures did not develop. However, callus originating in medium B retained morphogenetic capacity as was evidenced by subsequent shoot regeneration when they were transferred to medium A with K and NAA replaced by 1.0 mg/l zeatin (Z). The potential value of incorporating this regeneration trait into Lycopersicon species and cultivated lines for use in tissue culture programs is discussed.</div></front></TEI><affiliations><list><country><li>Philippines</li><li>États-Unis</li></country><region><li>Michigan</li></region><settlement><li>East Lansing</li></settlement><orgName><li>Université d'État du Michigan</li></orgName></list><tree><country name="États-Unis"><region name="Michigan"><name sortKey="Zapata, J" sort="Zapata, J" uniqKey="Zapata J" first="J." last="Zapata">J. Zapata</name></region><name sortKey="Sink, C" sort="Sink, C" uniqKey="Sink C" first="C." last="Sink">C. Sink</name></country><country name="Philippines"><noRegion><name sortKey="Zapata, J" sort="Zapata, J" uniqKey="Zapata J" first="J." last="Zapata">J. Zapata</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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